Electrochemical aptasensor for the detection of adenosine by using PdCu@MWCNTs-supported bienzymes as labels.

摘要: Abstract A highly sensitive electrochemical adenosine aptasensor was fabricated by covalently immobilizing 3′-NH 2 -terminated capture probe (SSDNA1) and thionine (TH) on Au–GS modified glassy carbon electrode. 3′-SH-terminated aptamer (SSDNA2) adsorbed onto palladium/copper alloyed supported MWCNTs (PdCu@MWCNTs)-conjugated multiple bienzymes, glucose oxidase (GOx), horseradish peroxidase (HRP) (SSDNA2/PdCu@MWCNTs/HRP/GOx). Then, it immobilized the electrode surface through hybridization between probe. The signal amplified based gradual electrocatalytic reduction of GOx-generated hydrogen peroxide HRP mediating ability loaded TH. However, peak current TH decreased in presence because interaction its made SSDNA2/PdCu@MWCNTs/HRP/GOx release from Various experimental parameters have been optimized for detection tests selectivity, reproducibility stability also performed. Under optimal condition, proposed displayed a wide linear range (10–400 nM) with low limit (2.5 nM), which has applied human serum samples satisfactory results. Thus, combination as sensor platform PdCu@MWCNTs/HRP/GOx labels can be promising amplification strategy detection.