Highly selective and sensitive detection of adenosine utilizing signal amplification based on silver ions-assisted cation exchange reaction with CdTe quantum dots
作者： Yueyue Song , Tang Tang , Xu Wang , Guanhong Xu , Fangdi Wei
DOI: 10.1016/J.SNB.2017.02.177
关键词:
摘要: Abstract Adenosine (AD) is a biomarker for monitoring the progress of lung cancer and its analysis in biological fluids patients significant. Here, we report novel signal amplification platform sensitive selective AD on basis silver ions-assisted cation exchange reaction with CdTe quantum dots (QDs). The aptamer was engineered to consist two pieces ssDNA which were respectively attached Fe 3 O 4 magnetic nanoparticles QDs. In presence AD, it would reassemble into intact tertiary structure, then form stable nanoparticle sandwich structure. After separation, final fluorescence process based twice reactions make detection more sensitive, compared directly measuring intensity exhibits excellent sensitivity high specificity, limit 0.217 nM AD. Moreover, present successfully applied determination urine samples range recoveries from 90％ 106％. results revealed that developed may provide promising analytical various target molecules biomedical field clinical diagnosis.
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