Cloning and characterization of the hemolysin determinants from Vibrio cholerae RV79(Hly+), RV79(Hly-), and 569B.

摘要: The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and nonhemolytic classical 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids detected with a 32P-labeled hly-specific DNA probe. Restriction endonuclease Sau3AI digestions hly loci two independently obtained RV79(Hly+) convertants, when compared digests loci, revealed that an apparent alteration (10 to 15 base pairs) had occurred. In contrast, 20-base-pair deletion was present in locus biotype V. 569B. Maxicell analysis immunoprecipitation labeled proteins E. which are encoded by nuclease BAL 31-deleted plasmids, as well [35S]methionine-labeled proteins, suggest hemolysin is 84,000-dalton polypeptide.