Identity of hemolysins produced by Vibrio cholerae non-O1 and V. cholerae O1, biotype El Tor.

摘要: Hemolysins purified from non-O1 Vibrio cholerae (non-O1 hemolysin) and a O1, biotype El Tor (El were investigated for their homology. The hemolysins isolated the culture supernatant fluids by ammonium sulfate precipitation gel filtration on Sephadex G-100 columns. gave single bands with an identical mobility conventional polyacrylamide disc electrophoresis. molecular weights of estimated to be about 60,000 sodium dodecyl sulfate-polyacrylamide electrophoresis, amino acid compositions very similar. specific activities identical, both neutralized same extent antisera against homologous heterologous hemolysins. Ouchterlony double immunodiffusion tests antihemolysin serum common (fused) precipitin line. These data indicate that hemolysin is biologically, physicochemically, immunologically indistinguishable hemolysin.