Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017

摘要: We have cloned the DNA encoding haemolysin of Vibrio cholerae El Tor strain 017 into plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI insert which leads to production in Escherichia coli K-12. Deletion analysis and transposon mutagenesis allowed us localize several regions affecting production. A number these mutants been analysed E. K-12 minicells. Three proteins identified: A, 80 kDal; B, 71 C, 22 kDal. is appears be cell-associated K-12, B C are required for its efficient suggest that genes designated hlyA, hlyB hlyC, respectively.