The "HemoQuant" test: a specific and quantitative determination of heme (hemoglobin) in feces and other materials.

摘要: We describe a new, specific, quantitative method for fecal blood, based on conversion of nonfluorescing heme to fluorescing porphyrins, that obviates serious deficiencies inherent in currently used tests. A two-reagent system is determine the two hemoglobin-related fractions are found feces. The hot citric acid extract includes only variable fraction porphyrins have been preformed from intestinal tract; this often major fraction. Total hemoglobin indirectly determined by reaction with heated oxalic acid:FeSO4 reagent, which converts remaining porphyrin without loss porphyrins. three-step purification procedure eliminates interfering materials. Analytical recovery added linearly related concentration over several-thousand-fold range. assay equally applicable whole blood or sub-microgram amounts 8-mg (wet weight) sample tested. Quality control liquid chromatographic and fluorometric analysis documents fluorescence specificity heme-derived