Population analysis of a commercial Saccharomyces cerevisiae wine yeast in a batch culture by electric particle analysis, light diffraction and flow cytometry.

摘要: Data from electric particle analysis, light diffraction and flow cytometry analysis provide information on changes in cell morphology. Here, we report analyses of Saccharomyces cerevisiae populations growing a batch culture using these techniques. The size distributions were determined by order to compare their outcomes. Flow parameters forward (related size) side granularity) scatter also complement this information. These yeast properties analysed statistically complexity index. at the lag phase was smaller than that beginning exponential phase, whereas during stationary converged with values observed phase. experimental techniques, when used together, allow us distinguish among characterize size, granularity structure population through three growth phases. patterns are better showing existence subpopulations different phases, especially use index context helped differentiate phases confirmed heterogeneity.