Analysis of marker expression in porcine cell lines derived from blastocysts produced in vitro and in vivo.

摘要: The present study was designed to extensively characterize cell lines derived from porcine blastocysts by several methodical approaches, including morphological observation, cytogenetic analysis, estimation of alkaline phosphatase activity and detection specific marker expression at the mRNA/protein level. A comparison made between properties isolated in vivo- vitro-obtained blastocysts. Our results showed that 57.1% vivo-obtained attached feeder layer 33.3% them started grow a monolayer. percentage vitro-produced lower (24.6%), only 6.9% grow. Outgrowths formed mainly trophectoderm or epithelial-like monolayer, whereas heterogeneous outgrowths also contained cells with embryonic stem (ES)-like morphology. Detailed analyses primary ES-like morphology expressed pluripotency markers OCT-4 NANOG revealed intensive staining, while they did not express differentiation. majority passaged cells, those morphology, lacked protein differentiation (cytokeratin 18, lamins A/C, transferrin, α-fetoprotein GATA-4), although still exhibited weak activity. Moreover, these spontaneously differentiated into neural, fibroblast even presence leukaemia inhibitory factor. show complex analysis as well is necessary for proper interpretation data studies.