Insertion of transposons through the major cotransduction gap of Escherichia coli K-12.

摘要: The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this which, by sequential transduction, link sbcA (min 29.8) with manA 35.7) and thus eliminate gap. These results indicate that length DNA in region, as measured is not significantly different obtained conjugational time entry. Since segment E. has few known genes, these transposon insertions will be useful for genetic manipulations region describe usefulness markers rapidly mapping mutations which may isolated min 27 37.