Molecular cloning and expression of hipA, a gene of Escherichia coli K-12 that affects frequency of persistence after inhibition of murein synthesis.

摘要: The hipA gene at 33.8 min on the Escherichia coli chromosome controls frequency of persistence upon inhibition murein synthesis; for strains bearing hipA+ is 10(-6), and hipA- 10(-2). hip+ has been cloned by selection a kanamycin resistance determinant 33.9 min. dominant over in both recA+ recA- backgrounds. smallest DNA insert which contains hipA+, as determined ability plasmids to complement strains, 1,885 base pairs. Both orientations are obtained when cloning site vector remote from strong promoters; hipA-, encode unique peptide 50,000 Mr. probable direction transcription deduced pattern peptides encoded either end adjacent sequences have deleted. This information recovery only one orientation close promoter suggest that high level expression not tolerated E. coli.