摘要: In this chapter we summarize briefly the use of cantilever sensors for pathogen detection. Both micro- and macro-cantilever have been investigated detecting pathogens in liquid samples. review examine previous work progress on using piezoelectric-excited millimeter-sized (PEMC) developed author’s laboratory. PEMC immobilized with an antibody specific to target has shown be very highly sensitive one cell per mL liter samples 10 cells 10,mL samples, both buffers at similar concentrations food matrices. After a brief introduction, physics sensing is reviewed, followed by characterization sensors, finally results from detection experiments are described.
springer.com
springerlink.com参考文章(54)
B. Ilic, H. G. Craighead, S. Krylov, W. Senaratne, C. Ober, P. Neuzil, Attogram detection using nanoelectromechanical oscillators Journal of Applied Physics. ,vol. 95, pp. 3694- 3703 ,(2004) , 10.1063/1.1650542
Kishan Rijal, Raj Mutharasan, Piezoelectric-excited millimeter-sized cantilever sensors detect density differences of a few micrograms/mL in liquid medium Sensors and Actuators B: Chemical. ,vol. 124, pp. 237- 244 ,(2007) , 10.1016/J.SNB.2006.12.043
B. Ilic, D. Czaplewski, H. G. Craighead, P. Neuzil, C. Campagnolo, C. Batt, Mechanical resonant immunospecific biological detector Applied Physics Letters. ,vol. 77, pp. 450- 452 ,(2000) , 10.1063/1.127006
Kavita Arora, Subhash Chand, B.D. Malhotra, Recent developments in bio-molecular electronics techniques for food pathogens. Analytica Chimica Acta. ,vol. 568, pp. 259- 274 ,(2006) , 10.1016/J.ACA.2006.03.078
Monika W. Oli, William P. McArthur, L. Jeannine Brady, A whole cell BIAcore assay to evaluate P1-mediated adherence of Streptococcus mutans to human salivary agglutinin and inhibition by specific antibodies Journal of Microbiological Methods. ,vol. 65, pp. 503- 511 ,(2006) , 10.1016/J.MIMET.2005.09.011
B. Ilic, D. Czaplewski, M. Zalalutdinov, H. G. Craighead, P. Neuzil, C. Campagnolo, C. Batt, Single cell detection with micromechanical oscillators Journal of Vacuum Science & Technology B. ,vol. 19, pp. 2825- 2828 ,(2001) , 10.1116/1.1421572
David Maraldo, Raj Mutharasan, Optimization of antibody immobilization for sensing using piezoelectrically excited-millimeter-sized cantilever (PEMC) sensors Sensors and Actuators B-chemical. ,vol. 123, pp. 474- 479 ,(2007) , 10.1016/J.SNB.2006.09.034
V. Koubová, E. Brynda, L. Karasová, J. Škvor, J. Homola, J. Dostálek, P. Tobiška, J. Rošický, Detection of foodborne pathogens using surface plasmon resonance biosensors Sensors and Actuators B: Chemical. ,vol. 74, pp. 100- 105 ,(2001) , 10.1016/S0925-4005(00)00717-6
Kishan Rijal, Angela Leung, P. Mohana Shankar, Raj Mutharasan, Detection of pathogen Escherichia coli O157:H7 AT 70 cells/mL using antibody-immobilized biconical tapered fiber sensors. Biosensors and Bioelectronics. ,vol. 21, pp. 871- 880 ,(2005) , 10.1016/J.BIOS.2005.02.006
Andrew J. Detzel, Gossett A. Campbell, Raj Mutharasan, Rapid assessment of Escherichia coli by growth rate on piezoelectric-excited millimeter-sized cantilever (PEMC) sensors Sensors and Actuators B-chemical. ,vol. 117, pp. 58- 64 ,(2006) , 10.1016/J.SNB.2005.10.045