Detection of foodborne pathogens using surface plasmon resonance biosensors

作者: V. Koubová , E. Brynda , L. Karasová , J. Škvor , J. Homola

DOI: 10.1016/S0925-4005(00)00717-6

关键词: Surface plasmonListeria monocytogenesSalmonellaListeriaBiosensorSurface plasmon resonanceChemistrySalmonella enteritidisChromatographyAnalytical chemistryInstrumentation (computer programming)Electrical and Electronic EngineeringMaterials ChemistryElectronic, Optical and Magnetic MaterialsSurfaces, Coatings and FilmsCondensed matter physicsMetals and Alloys

摘要: Abstract Salmonella enteritidis and Listeria monocytogenes were detected in real time using a surface plasmon resonance (SPR) sensor based on prism excitation of plasmons spectral interrogation. The respective antibodies against the pathogens immobilized gold as covalently crosslinked double-layer or bound albumin layer. could be by at concentrations down to 10 6  cell/ml. sensitivity was comparable with that standard ELISA which same used.

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