The primary DNA sequence determines in vitro methylation by mammalian DNA methyltransferases.

摘要: Publisher Summary This chapter discusses the mechanism by which mammalian DNA methyltransferases recognize and modify DNA. The studies described in used that were purified 800-fold from nuclear extracts of HeLa S3 cells 115- fold mouse erythroleukemia (MEL) cell nuclei. strategy purification procedure was designed to produce niethyltransferase preparations virtually free deoxyribonuclease activity. Correlations between level methylation specific genomic sequences expression certain genes differentiating tissues suggest is one possible mechanisms for control gene expression. If a primary event regulation expression, elucidation complex factor(s) may play important role(s) would facilitate an understanding use defined oligodeoxynucleotides synthetic polydeoxyribonucleotides as substrates enzyme has facilitated identification various factors influence rate specificity vitro.