Chapter 9 – Molecular Diagnostic Techniques and Other Tests for Direct Detection of HIV

摘要: Serologic testing has been greatly refined, vastly improving the sensitivity and specificity of such testing. HIV antigen culture techniques have become useful in certain clinical situations. Finally, polymerase chain reaction (PCR) allowed direct detection both complementary DNA (cDNA) as well RNA. Plasma viral-RNA measurement, by PCR or other methodologies, an essential component care, particularly when implemented a prognostic tool. Furthermore, p24 is HIV-1 core protein encoded “gag” gene. It expressed soon after acquisition antibody to forms shortly thereafter. This can be detected serum, plasma, cerebrospinal fluid. Measurement serum may clinically HIV-1-infected patients prior seroconversion. The chapter also discusses branched (bDNA) assay for measurement plasma After HIV-1, RNA from patient captured on microplate, probes are hybridized specific gene segments baound RNA, thereby amplifying signal. A chemiluminescent then used quantitate amount sample.