An in Situ Intracellular Self-Assembly Strategy for Quantitatively and Temporally Monitoring Autophagy.

摘要: Autophagy plays a crucial role in the metabolic process. So far, conventional methods are incapable of rapid, precise, and real-time monitoring autophagy living objects. Herein, we describe an situ intracellular self-assembly strategy for quantitative temporal determination objectives. The intelligent building blocks (DPBP) composed by bulky dendrimer as carrier, bis(pyrene) derivative (BP) signal molecule, peptide linker responsive unit that can be cleaved autophagy-specific enzyme, i.e., ATG4B. DPBP maintains quenched fluorescence with monomeric BP. However, is specifically tailored upon activation autophagy, resulting self-aggregation BP residues which emit 30-fold enhanced fluorescence. By measuring intensity fluorescent signal, able to quantitatively evaluate autophagic level. In comparison traditional techniques, such TEM, Western blot, GFP-LC3, reliability ...