Nanoimprinted thin films of reactive, azlactone-containing polymers: combining methods for the topographic patterning of cell substrates with opportunities for facile post-fabrication chemical functionalization.

摘要: Approaches to the fabrication of surfaces that combine methods for topographic patterning soft materials with opportunities facile, post-fabrication chemical functionalization could contribute significantly advances in biotechnology and a broad range other areas. Here, we report can be used introduce well-defined nano- microscale features thin films reactive polymers containing azlactone functionality using nanoimprint lithography (NIL). We demonstrate NIL imprint patterns into poly(2-vinyl-4,4-dimethylazlactone) copolymer methyl methacrylate 2-vinyl-4,4-dimethylazlactone silicon masters having grooves ridges ranging width from 400 nm 2 microm, demonstrating potential this method transfer these over feature sizes densities. further survives temperatures pressures associated NIL, topographically patterned readily functionalized by treatment surface-accessible small molecules primary amines. The results experiments which NIH-3T3 cells were seeded onto imprinted lined pitch 4 microm demonstrated attach proliferate on azlactone-containing they align direction pattern. Finally, amine-functionalized poly(ethylene glycol) (PEG) create regions prevent cell adhesion. study suggest approaches (e.g., peptides, proteins, carbohydrates, etc.) biotechnological interest. ability manipulate define both physical topography provide investigate combined effects substrate behavior may also useful applications.