Polyethylene Glycol Coatings on Plastic Substrates for Chemically Defined Stem Cell Culture

摘要: Human mesenchymal stem cells (hMSCs) are a widely available and clinically relevant cell type with host of applications in regenerative medicine. Current clinical expansion methods can lead to selective changes hMSC phenotype potentially resulting from relatively undefi ned culture surfaces. Chemically defi synthetic surfaces aid understanding the infl uence cell‐material interactions on behavior. Here, thin copolymer coating for plastic substrates is developed. The random synthesized by living free radical polymerization characterized solution before application substrate, ensuring homogeneous limiting sample-to-sample variations. ability coat multiple substrate types cover large surface areas reported. Arg‐Gly‐Asp-containing peptides incorporated into under aqueous conditions via their lysine or cysteine side chains, amide thioester linkages, respectively. Stability studies show linkages be stable labile standard serum-containing conditions. In addition, chemically passaging hMSCs using only ethylenediaminetetraacetic acid polystyrene dishes shown. After passage, seeded back onto same plate, indicating potential reusability coating.