Improved detection of microbial DNA after bead-beating before DNA isolation.
作者： Richard de Boer , Remco Peters , Sonja Gierveld , Tim Schuurman , Mirjam Kooistra-Smid
DOI: 10.1016/J.MIMET.2009.11.009
关键词:
摘要: Abstract Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis cell walls all possible microorganisms. We demonstrated that physical disruption bead-beating 0.1 mm beads combination MagNA Pure III enhances diverse Gram-positive microorganisms and may be used to optimize protocols routine diagnostics.
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