Type IV collagenases in human amniotic fluids and amnion epithelial cells
作者： Johanna Lehtovirta , Tapio Vartio
DOI: 10.1016/0167-4838(94)90075-2
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摘要: Abstract Gelatin zymograms revealed in human second trimester amniotic fluids a constant M r 66000 proteolytically active polypeptide that immunoblotting was recognized by antiserum against the fibroblast gelatinase/type IV collagenase. Isolated cultured amnion epithelial cells produced proteinase and frequently 92000 one. The production of former enhanced transforming growth factor-β latter tetradecanoyl phorbol acetate. Immunoprecipitation experiments indicated cell identical with round one corresponded to macrophage/granulocyte Approximate quantifications 66 000 sera from same individuals were assessed comparison dilution series samples standard enzyme preparations zymography. These measurements amount about 5–10 μg/ml representing 0.1–0.2% total proteins. In corresponding figures 30–40 0.03–0.06%, respectively. Thus relative concentration approx. 3–4-fold sera, suggesting local protein. may be under so far unknown physiologic stimulus also start secreting enzyme.
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