摘要: … of computational enzyme methods is that a high-throughput assay is not strictly necessary for enzyme design. … are employed in addition to computational design, a medium to high-…
Daniela Röthlisberger, Olga Khersonsky, Andrew M. Wollacott, Lin Jiang, Jason DeChancie, Jamie Betker, Jasmine L. Gallaher, Eric A. Althoff, Alexandre Zanghellini, Orly Dym, Shira Albeck, Kendall N. Houk, Dan S. Tawfik, David Baker, Kemp elimination catalysts by computational enzyme designNature. ,vol. 453, pp. 190- 195 ,(2008) , 10.1038/NATURE06879