Real-time PCR machine system modeling and a systematic approach for the robust design of a real-time PCR-on-a-chip system.

摘要: Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue normal operation, this study presents system model of the real-time Polymerase Chain Reaction (PCR) to analyze instrument design through numerical experiments. Based on analysis, systematic approach was developed lower variation achieve robust PCR-on-a-chip system. Accelerated lift testing adopted evaluate chip prototype. According life test plan, proposed simulated work continuously over three years with similar reproducibility quantification. This only shows robustness lab-on-a-chip system, but also verifies effectiveness our method achieving design.