Expression of selected human HOX-2 genes in B/T acute lymphoid leukemia and interleukin-2/interleukin-1 beta-stimulated natural killer lymphocytes.

摘要: Although the key role of human homeobox (HOX) genes in development is well established, their function adult cells still under scrutiny. We have analyzed, normal blood cell subpopulations, acute lymphoid leukemia (ALL) lines, and primary blasts, RNA expression all HOX-2 cluster (5′-2.5, 2.4, 2.3, 2.2, 2.1, 2.6, 2.7, 2.8, 2.9, 3′) nine HOX-1, -3, -4 by Northern blotting, RNAse protection, and/or reverse transcriptase polymerase chain reaction (RT-PCR). The analyzed were never expressed tested populations. Natural killer (NK) activated interleukin-2 (IL-2)/IL-1 beta-treated cultures exhibit a gradually increasing, abundant three (2.2, 2.8), while other (2.3, 2.7) are at lower level late culture times. However, no gene quiescent lymphocytes (NK, B T [T-cell receptor (TCR) alpha/beta, gamma/delta lymphocytes, thymocytes] cells), granulocytes, monocytes. In B- T-ALL according to different patterns: (1) widespread transcription (seven genes, including 2.3 2.6) Peer line bearing TCR gamma/delta; (2) 2.5, 2.6 SEZ 627 line, which derives from an HTLV-1+ T-helper leukemia; (3) both CEM four ALL lines (interestingly, CALLA- B-ALL constantly 2.3/2.6 RNA+); (4) was detected one T- two lines. Primary blasts five pre-B-ALL showed selective or more namely 2.7. Our data compatible with hypothesis that selected HOX- 2 play IL-2/IL-1 beta-induced activation proliferation NK possibly oncogenetic process some B-ALL.