Development of a DNA Microarray for Enterococcal Species, Virulence, and Antibiotic Resistance Gene Determinations among Isolates from Poultry

摘要: A DNA microarray (Enteroarray) was designed with probes targeting four species-specific taxonomic identifiers to discriminate among 18 different enterococcal species, while other were identify virulence factors and 174 antibiotic resistance genes. In total, 262 genes utilized for rapid species identification of isolates, characterizing their potential through the simultaneous endogenous Enterococcal isolates from broiler chicken farms initially identified by using API 20 Strep system, results compared those obtained atpA, recA, pheS, ddl represented on our microarray. Among 171 studied, five system: Enterococcus faecium, E. faecalis, durans, gallinarum, avium. The Enteroarray detected same as Strep, well two more: casseliflavus hirae. Species comparisons resulted in 15% (27 isolates) disagreement between methods identifiable 24% (42 when considering seven species. specificity key consistent literature adding further robustness redundant probe data. Sequencing cpn60 gene confirmed complete accuracy results. new should prove be a useful tool accurately genotype strains enterococci assess potential.