Non-syntenic amplification of MDM2 and MYCN in human neuroblastoma.

摘要: Amplification of the MYCN gene is a well documented genetic alteration aggressively growing human neuroblastomas. Through cytogenetic studies we have identified neuroblastoma cell lines which, in addition to amplified MYCN, carry DNA not harbouring MYCN. In situ hybridization biotinylated total genomic metaphase chromosomes normal lymphocytes by reverse revealed be derived from chromosome 12 band q13-14. Subsequent filter analyses showed 20- 40-fold amplification MDM2 gene, located at 12q13-14, both three and an original tumor, As apparent consequence abundant protein was present, part which complexed with p53.