Comparative proteomic investigation of Marsupenaeus japonicus hepatopancreas challenged with Vibrio parahaemolyticus and white spot syndrome virus
作者： Xianyun Ren , Yunbin Zhang , Ping Liu , Jian Li
DOI: 10.1016/J.FSI.2019.08.039
关键词:
摘要: This study aimed to use isobaric tags (IBTs) investigate the immune response of hepatopancreas Marsupenaeus japonicas infected with Vibrio parahaemolyticus or white spot syndrome virus (WSSV). Liquid chromatography-tandem mass spectrometry and protein sequencing identified 1005 proteins. Among them, 109 proteins were upregulated 94 downregulated after V. infection. After WSSV infection, 130 as differentially abundant, including 88 that 42 downregulated. Fifty-four abundant both A number related cytoskeletal processes, actin myosin, apoptosis-related in shrimp indicating phagocytosis apoptosis may be involved Quantitative real-time PCR was carried out verify reliability proteomic data. These data provide a basis characterize immunity-related processes infection parahaemolyticus.
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