Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction.
作者： Astrid Vabret , Franck Mouthon , Thomas Mourez , Stephanie Gouarin , Joëlle Petitjean
DOI: 10.1016/S0166-0934(01)00343-3
关键词:
摘要: An RT-PCR-hybridization was developed that amplified genetic material from the M protein gene of HCoV-229E and HCoV-OC43. The analytic sensitivity these original primers were compared with defined in N described previously. results show 0.05 TCID50 0.01 HCoV-OC43 can be detected by this molecular method using method. Detection clinical specimens is possible method: 348 respiratory (202 sputum 146 nasal aspirates) tested 12 human coronavirus are (3%). could provide a useful tool for demonstrating role infections tract.
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