Diagnosis of human viruses by polymerase chain reaction technology

摘要: The basis for the effective treatment and cure of a patient is rapid diagnosis disease its causative agent, which based on analysis clinical symptoms coupled with laboratory tests. Although advance ments have been made in virus diseases, neces sary isolation from specimens relatively long procedure. Viruses integrate into cellular DNA (such as human immunodeficiency virus, HIV -1, or hepatitis B virus) are difficult to identify by molecular techniques, while viruses exist material low concentrations even more formidable identify. Recently, application polymerase chain reaction (peR) technique developed K. D. Mullis detailed study Saiki et al. (1985) led revolution diagnosis. peR was rapidly applied material. Volume 1 Frontiers Virology provides new information advan tages use many disease-causing viruses, well some problems use."