Detection and quantitation of HBV DNA by semi-nested PCR in donated blood: comparison with HBV serological markers.
作者： Chieko Matsumoto , Kusuya Nishioka , Takashi Oguchi , Shigeki Mitsunaga , Noriyuki Nojiri
DOI: 10.1016/S0166-0934(97)00037-2
关键词:
摘要: To detect and quantitate hepatitis B virus (HBV) DNA, semi-nested polymerase chain reaction (PCR) method was designed for amplifying the HBV core region DNA. Cloned DNA used as a quantitation control, upon electrophoresis of PCR product, one, two, or three bands amplified were observed using small ( = 1250 mol) quantity template respectively. Using this method, quantitated in donated blood tested serological markers. Most surface antigen (HBsAg) high titer samples showed on electrophoresis, indicating level while most HBsAg low one band, detected 7 out 36 HBsAg-undetectable anti-HBc-positive (19.4%) but all In almost e antigen-positive high,
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