Evaluation of a Hypocrea jecorina Enzyme Preparation for Hydrolysis of Tifton 85 Bermudagrass

摘要: Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres USA for hay and forage. Of bermudagrass cultivars, exhibits improved digestibility because ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techniques. A previously pressurized batch hot water (PBHW) method was used treat enzymatic hydrolysis. Native grass (untreated) PBHW-pretreated material were compared as substrates fungal cultivation produce enzymes. Cellulase activity, measured via filter paper assay, higher fungi cultivated grass, whereas other nine enzyme assays produced activities untreated grass. Ferulic acid vanillin levels increased significantly preparations release these compounds may have contributed observed reduction activities. Culture supernatant from bermudagrass-grown combined with two commercial activity profiles are reported. The amount reducing sugar liberated by mixture Hypocrea jecorina (after 192 h incubation bermudagrass) individually or combination feruloyl esterase 72.1 84.8%, respectively, cellulase preparation analyzed under same conditions.