Preserving Proteins Under High Pressure and Low Temperature
作者： Takahiro Takekiyo , Minoru Kato , Yukihiro Yoshimur
DOI: 10.5772/37131
关键词:
摘要: Our group has a continuing interest in devising new freezing-protection agents. To this end, we have systematically investigated the structure of aqueous electrolyte salt solutions and room-temperature ionic liquids (RTILs), which consist cations anions. We found that such water-mixed remain liquid even at 1.0 GPa 77 K (achieved by rapid cooling 500 K/min) (Imai et al., 2010; Takekiyo 2006a, 2006b; Yoshimura 2009, 2011) water-soluble RTILs as 1-butyl-3-methylimidazolium tetrafluoroborate 2.0 2011, 2011). These results suggest high pressure low temperature use RTILs-water mixtures might be useful for freezing preservation protein solutions.
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