Identification and purification of two distinct complexes containing the five RAD51 paralogs.

作者: Jean-Yves Masson , Madalena C Tarsounas , Alicja Z Stasiak , Andrzej Stasiak , Rajvee Shah

DOI: 10.1101/GAD.947001

关键词:

摘要: Cells defective in any of the RAD51 paralogs (RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3) are sensitive to DNA cross-linking agents ionizing radiation. Because required for assembly damage-induced foci, mutant cell lines homologous recombination show genomic instability, their defect is thought be caused by an inability promote efficient recombinational repair. Here, we that five exist two distinct complexes human cells: one contains RAD51B, XRCC2 (defined as BCDX2), whereas other consists RAD51C with XRCC3. Both protein have been purified homogeneity biochemical properties investigated. BCDX2 binds single-stranded gaps duplex DNA, accord proposal play early (pre-RAD51) role Moreover, complex specifically nicks DNA. We suggest extreme sensitivity paralog-defective owing defects processing incised cross links consequential failure initiate repair at these sites.

参考文章(64)
F M Sladek, M M Munn, W D Rupp, P Howard-Flanders, In vitro repair of psoralen-DNA cross-links by RecA, UvrABC, and the 5'-exonuclease of DNA polymerase I. Journal of Biological Chemistry. ,vol. 264, pp. 6755- 6765 ,(1989) , 10.1016/S0021-9258(18)83494-9
Akira Shinohara, Tomoko Ogawa, Stimulation by Rad52 of yeast Rad51- mediated recombination Nature. ,vol. 391, pp. 404- 407 ,(1998) , 10.1038/34943
F.E. Benson, A. Stasiak, S.C. West, Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA. The EMBO Journal. ,vol. 13, pp. 5764- 5771 ,(1994) , 10.1002/J.1460-2075.1994.TB06914.X
Michael M. Cox, Myron F. Goodman, Kenneth N. Kreuzer, David J. Sherratt, Steven J. Sandler, Kenneth J. Marians, The importance of repairing stalled replication forks Nature. ,vol. 404, pp. 37- 41 ,(2000) , 10.1038/35003501
James H. New, Tomohiko Sugiyama, Elena Zaitseva, Stephen C. Kowalczykowski, Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A Nature. ,vol. 391, pp. 407- 410 ,(1998) , 10.1038/34950
Fiona E. Benson, Peter Baumann, Stephen C. West, Synergistic actions of Rad51 and Rad52 in recombination and DNA repair. Nature. ,vol. 391, pp. 401- 404 ,(1998) , 10.1038/34937
Galina Petukhova, Sabrina Stratton, Patrick Sung, Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins Nature. ,vol. 393, pp. 91- 94 ,(1998) , 10.1038/30037
Joanna S. Albala, Michael P. Thelen, Christa Prange, Wufang Fan, Mari Christensen, Larry H. Thompson, Gregory G. Lennon, Identification of a novel human RAD51 homolog, RAD51B. Genomics. ,vol. 46, pp. 476- 479 ,(1997) , 10.1006/GENO.1997.5062
Stefan Sigurdsson, Kelly Trujillo, BinWei Song, Sabrina Stratton, Patrick Sung, Basis for avid homologous DNA strand exchange by human Rad51 and RPA. Journal of Biological Chemistry. ,vol. 276, pp. 8798- 8806 ,(2001) , 10.1074/JBC.M010011200