Expression of the leukocyte functional molecule (LFA-1) on mouse platelets.

摘要: Platelet involvement in adhesion, hemostasis, and immune adherence is mediated by functionally associated cell surface molecules. Platelets are also involved cytolytic reactions, but little known about the mechanisms or biologic significance of these processes. To further investigate molecules concerned with platelet function, antisera against mouse platelets, thymocytes, macrophages monoclonal antibodies Mac-1 (complement receptor type 3) leukocyte function-associated glycoprotein 1 (LFA-1) were used to demonstrate LFA-1--like on platelets. The alpha subunits thymocyte LFA-1 showed identical electrophoretic mobility differed significantly from subunit macrophage Mac-1. Peptide mapping demonstrated identity beta three that was distinct LFA-1. LFA-1, as iodination lactoperoxidase labeling sialic acid residues sodium borohydride, not a major component membrane. functional platelets has yet be demonstrated, having effect adenosine diphosphate-induced aggregation adherence. In contrast, although could immunoprecipitation studies, its presence clearly low levels antibody binding enzyme-linked immunosorbent assays ability M1/70 inhibit Human which inactive assays, shown lack