Adipose tissue engineering in vivo with adipose-derived stem cells on naturally derived scaffolds.
作者： L. Flynn , G. D. Prestwich , J. L. Semple , K. A. Woodhouse
DOI: 10.1002/JBM.A.32044
关键词:
摘要: Placental decellular matrix (PDM) and PDM combined with cross-linked hyaluronan (XLHA) scaffolds, seeded primary human adipose-derived stem cells (ASC), were investigated in a subcutaneous athymic mouse model. The vivo response at 3 8 weeks was characterized using histological immunohistochemical staining. Fibrous capsule formation assessed the relative number of adipocytes each scaffold quantified. Undifferentiated ASC localized immunostaining for vimentin. Unilocular multilocular identified by intracellular lipid accumulation. Staining murine CD31 implant vascularization. Both scaffolds macroscopically maintained their three-dimensional volume supported mature adipocyte populations vivo. There evidence integration host contribution to adipogenic response. results suggested that incorporating XLHA had positive effect terms angiogenesis adipogenesis. Overall, showed great promise adipose tissue regeneration.
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