Novel methods for biocatalyst screening.
作者： Denis Wahler , Jean-Louis Reymond
DOI: 10.1016/S1367-5931(00)00184-8
关键词:
摘要: There have been a number of recent advances in catalysis assays applicable for screening biocatalyst libraries high-throughput format. These include instrumental such as high-performance liquid chromatography, mass spectrometry, capillary electrophoresis and IR-thermography, reagent-based producing spectroscopic signals (UV/VIS or fluorescence) response to reaction progress, based on fluorogenic chromogenic substrates. substrates enable the assaying variety enzymes enantioselective stereoselective manner, including alcohol dehydrogenases, aldolases, lipases, amidases, epoxide hydrolases phosphatases.
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