Construction of Conditional Knockdown Mutants in Mycobacteria
作者： Dirk Schnappinger , Kathryn M. O’Brien , Sabine Ehrt
DOI: 10.1007/978-1-4939-2450-9_9
关键词:
摘要: By definition, essential genes are fundamental to bacterial growth, yet the functions of many such remain unknown. Essential furthermore central activity most antibacterial drugs and among attractive targets for development new therapeutics. This chapter describes how synthetic genetic switches that utilize transcriptional repression, controlled proteolysis, or both silence gene can be applied construct characterize conditional knockdown (cKD) mutants in Mycobacterium smegmatis tuberculosis.
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