Changes in plasma membrane glycoproteins of rat spermatozoa during maturation in the epididymis.

摘要: Glycoproteins on the plasma membrane of testicular and cauda epididymidal spermatozoa have been labeled with galactose oxidase/NaB [3H]4 sodium metaperiodate/NaB[3H]4, followed by analysis SDS polyacrylamide gels. The major glycoprotein labeling has a molecular weight 110,000 whereas greater than 90% radio-label is incorporated into proteins 32,000. These 32,000-mol wt X are homologous similar purified from epididymal secretion which shown previously to be synthesized in caput epididymidis under hormonal control. Immunofluorescence revealed that present flagellum mature but not immature they patchy distribution suggesting mobile within plane membrane. membrane-bound possess hydrophobic domains as charge-shift electrophoresis also label lipophilic photoaffinity probe contact lipid bilayer. evidence indicates there considerable reorganization structure during maturation epididymis some changes brought about direct interaction secretory proteins.