Repair of large full-thickness articular cartilage defects by transplantation of autologous uncultured bone-marrow-derived mononuclear cells.

摘要: The objective of this study was to investigate the feasibility autologous uncultured bone marrow-derived mononuclear cell transplantation in large full-thickness cartilage regeneration. After fixing with a hinged external fixator, entire surface left tibial plateau resected and defects were formed 48 rabbits. Animals divided into four groups: cells fibrin gel (BMC), peripheral blood-derived (PBC), alone (GEL), or nothing (CON) transplanted articular cavity 7 days after operation. rabbits killed 8 12 weeks repair investigated histologically scored using histological histochemical grading scale that modified from International Cartilage Repair Society Visual Histological Assessment Scale. To evaluate regenerated cartilage, we also morphometrically measured staining area positive for Safranin-O type II collagen calculated percentages areas respect soft tissue area. findings showed BMC group had superior compared other groups, PBC CON better than did GEL group. scores morphometrical measurements same results quantitively. contributes repair. easy safe method used is potentially viable clinical application.