Overcoming Expression and Purification Problems of RhoGDI Using a Family of “Parallel” Expression Vectors☆
作者： Peter Sheffield , Sarah Garrard , Zygmunt Derewenda
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摘要: We describe the construction of expression vectors based on three most frequently used gene fusion affinity tags [glutathione S-transferase (GST), maltose binding protein (MBP), and His6 peptide]. The polylinkers pGEX4T1, pMal-c2, a pET vector were replaced with polylinker isolated from baculovirus plasmid pFastBac. Once appropriate restriction sites have been introduced into gene, it can be fused to all little effort, allowing expression-screening experiments performed efficiently. discuss development use these respect overcoming purification problems encountered for RhoA GDP/GTP nucleotide dissociation inhibitor (RhoGDI) their advantages over commercially available vectors.
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