Insight into the molecular evolution of non-specific lipid transfer proteins via comparative analysis between rice and sorghum.

摘要: Phylogenetic analysis was conducted on 9 kDa non-specific lipid transfer protein (nsLTP) genes from nine plant species. Each of the five classified types in angiosperms exhibited eight conserved cysteine patterns. The most abundant nsLTP fell into type I category, which particularly enriched a grass-specific lineage clade I.1. Six pairs tandem copies distal region rice chromosomes 11 and 12 were well-preserved under concerted evolution, not observed sorghum. transgenic promoter‐reporter assay revealed that both sorghum displayed relatively expression feature epidermis growing tissue, supporting its functional roles cutin synthesis or defence against phytopathogens. For I, frequent stigma seed are indicative involvement pistil‐pollen interactions development. By way contrast, several V observed, mainly vascular bundle rosette as well young shoots, might be related with tissue differentiation signalling. Compared sorghum, highly redundant tissue-specific pattern among members I.1 suggests evolution via gene conversion favours preservation crucial motifs homogenization proximal promoter sequences high selection constraints. However, extensive regulatory subfunctionalization also have occurred relative low constraints, resulting divergence at level.