Identification and characterization of a ran gene promoter in the protozoan pathogen Giardia lamblia.

摘要: The promoter elements that regulate transcription initiation in Giardia lamblia are poorly understood. In this report, the of ran gene was studied using a luciferase expression plasmid pRANluc+ to monitor efficiency. An AT-rich sequence spanning -51/-20 relative translation start site identified and found be required for efficient by deletion mutation mapping pRANluc+. also sufficient activity as revealed from studies on 32-base pair synthetic derived region. Deletion two minimal elements, -51/-42 -30/-20, 6- 30-fold above background, respectively. sites luc+ messenger RNA were determined position plasmids shown primer extension experiments. Results electrophoretic mobility shift assays multiple DNA-protein complexes upon binding nuclear proteins with either DNA strand but not double-stranded promoter. Our results delineate first (ran), which provides an excellent model future regulation protozoan parasite.