The Giardia lamblia genome.

摘要: Giardia lamblia is a protozoan parasite of humans and other mammals that thought to be one the most primitive extant eukaryotic organisms. Although distinctly eukaryotic, it notable for its lack mitochondria, nucleoli, perixosomes. It has been suggested spp. are pre-mitochondriate organisms, but identification genes in G. mitochondrial origin generated controversy regarding designation. Giardi trophozoites have two nuclei identical all ways studied. They polyploid with at least four, perhaps eight or more, copies each five chromosomes per organism an estimated genome complexity 1.2x10(7)bp DNA, GC content 46%. There evidence recombination telomeres some chromosomes, multiple size variants single identified within cloned isolates. However, internal regions demonstrate no recombination. For example, there control vsp gene expression by DNA recombination, rapid mutation genes. Single pass sequences approximately 9% already obtained. An ongoing project plans obtain 95% random approach, as well complete physical map using bacterial artificial chromosome library. The results will facilitate better understanding biology their phylogenetic relationship