Oral sampling and human papillomavirus genotyping in HIV-infected patients.

摘要: J Oral Pathol Med (2012) 41: 288–291 Background:  human papillomavirus (HPV) is associated with several health complications especially in combination HIV infections. Screening may be useful, but methodologies and results have varied widely previous studies. We conducted a pilot study an HIV-positive population to evaluate HPV detection four different oral sample types. Methods:  Upon enrollment, oral-rinse (OR) was collected 10 ml saline. Additional samples of the buccal mucosa, tonsils, lesion if present were cytology brushes. DNA extracted using LC-MagNAPure, Linear Array genotyping Assay (Roche) used for genotyping. Results:  In from 100 participants, detected 39 (%) rinses, 13 mucosal 11 (12.9%) tonsil brushings. Of seven brushings collected, positive. All participants mucosal, tonsil, or also positive OR sample. Among rinse samples, 27 genotypes HPV84 (n = 6), HPV55 (n = 5), HPV83 (n = 5) being most common. Multiple infections 17 (range 2–9, mean 1.9 types). As potential cofactors, only receptive sex significantly (P = 0.018, odds ratio 2.9, 95% CI 1.2–6.9). Conclusion:  Sampling significant factor prevalence provides best representation cavity. To cofactors other than sex, larger studies case–control design are necessary.