The detection of Yersinia enterocolitica in biological samples by RT-PCR technique.

摘要: The research has been performed in order to optimize Yersinia enterocolitica diagnosis from blood samples and other biological different human clinical cases. Following this objective, the general technique of collecting processing was adapted and optimized, raising rapidity diagnosis. There have artificially contaminated different types with enterocolitica stems, checking efficiency accuracy optimized method. In finally developed a methodology identification naturally contaminated samples. As target-gene there used chromosome gene ail, this being present pathogenic strains Y. enterocolitica. sample marked at 5’ end with a report dyeing solution 3’ an extinction one. reading the RT-PCR, work protocol consisting 2 stages, including 45 cycles. This qualitative method demonstrated some specific results high sensitivity, positive ail detection rate Y. enterocolitica 267 type products, being 82 % using PCR and 56 culture methods.