High‐throughput 3D bioprinting of corneal stromal equivalents

摘要: In this study we designed high-throughput 3D bioprinting of corneal equivalents which may address the need for in vitro models. our digital cornea model, average dimensions adult were converted to shapes, then G-code files printed by BIOX printer (CELLINK). To maintain curvature cornea, a support scaffold was using stereolithographic printer. The could facilitate printing 6-12 corneas at time thus enabling printing. Human keratocytes (HCKs) incorporated optimized bio-ink, and cell-laden stromal printed. Printed structures cross-linked calcium chloride 100 mM, washed with Hanks' Balanced Salt Solution incubated 37°C fibroblast media. analyzed live dead assay, Alamar expression fibronectin actin green markers. able their structure, integrity, clarity. Live assay demonstrated that HCKs maintained high viability (>95%) 2 weeks. showed green. conclusion, fabrication combination stereolithography printing, extrusion based micro-transfer molding techniques achieved.