Genetic heterogeneity revealed by sequence analysis of Mycobacterium tuberculosis isolates from extra-pulmonary tuberculosis patients

摘要: Tuberculosis remains a major public health problem. Clinical tuberculosis manifests often as pulmonary and occasionally extra-pulmonary tuberculosis. The emergence of drug resistant tubercle bacilli its association with HIV is formidable challenge to curb the spread There have been concerted efforts by whole genome sequencing bioinformatics analysis identify genomic patterns establish relationship between genotype organism clinical manifestation Extra-pulmonary TB constitutes 15–20 percent total cases reported among immunocompetent patients, whereas patients incidence more than 50 percent. Genomic M. isolates from extra has not explored. DNA 5 derived cerebrospinal fluid, lymph node fine needle aspirates (FNAC) / biopsies, were sequenced. Next generation approach (NGS) was employed Single Nucleotide Variations (SNVs) computational methods used predict their consequence on functional genes. Analysis distribution SNVs led finding that there are mixed genotypes in patient many likely influence either gene function or expression. Phylogenetic correlated origin isolates. In addition, insertion sites IS elements identified revealed variation number position element compared reference H37Rv strain. results suggest NGS able small variations genomes including changes sites. Moreover, non-pulmonary documented. our indicates heterogeneity