Role of Vibrio cholerae neuraminidase in the function of cholera toxin.

摘要: Vibrio cholerae neuraminidase (NANase) is hypothesized to act synergistically with cholera toxin (CT) and increase the severity of a secretory response by increasing binding penetration CT enterocytes. To test this hypothesis, NANase gene (nanH) from V. Ogawa 395 was first cloned sequenced. Isogenic wild-type NANase- strains were then constructed using suicide vector-mediated mutagenesis. The influence on examined in vitro culture filtrates these isogenic strains. Fluorescence due fluorescein-conjugated C57BL/6 C3H mouse fibroblasts exposed NANase+ increased five- eightfold, respectively, relative that filtrates. In addition, short-circuit current measured Ussing chambers 65% filtrates, although difference decreased as production increased. role pathogenesis vivo intragastric inoculation into CD1 suckling mice. No fluid accumulation ratios seen at doses 10(4) 10(8) CFU, but produced 18% higher 10(9) CFU than when inoculated nonfasted It concluded plays subtle significant uptake susceptible cells under defined conditions.