Titanium dioxide ceramics control the differentiated phenotype of cardiac muscle cells in culture

摘要: A new approach, the cultivation of heart muscle cells on biocompatible scaffolds made from titanium dioxide ceramics was established to provide a mechanism for in vitro engineering vital tissue. Terminally differentiated ventricular myocytes isolated hearts adult rats were kept primary culture long periods time and used as an experimental model. The microenvironmental properties helped maintain tissue-like structural organisation cardiac vitro. Coating cell substrata with fine-grained imitating surface topography favoured formation focal adhesion complexes ventral plasma membrane cardiomyocytes. It also promoted cellular expression vinculin, protein that connects ECM integrin receptors network cytoplasmic filaments, which define shape. This topographical reinforcement cell-material interactions led stabilisation molecular linkage between extracellular contacts intracellular cytoskeleton thus assisted preservation maintenance phenotype long-term culture. results this work demonstrate promising pathway regulation by local geometric control.