Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells.

摘要: C-rich DNA has the capacity to form a tetra-stranded structure known as an i-motif. The i-motifs within genomic have been proposed contribute regulation of transcription. However, direct experimental evidence for existence these structures invivo missing. Whether i-motif in complex environment living cells is not currently known. Herein, using state-of-the-art in-cell NMR spectroscopy, we evaluate stabilities cellular environment. We show that formed from naturally occurring sequences human genome are stable and persist nuclei cells. Our data generally distinct those invitro. results first interlink stability invitro with their provide essential information design development i-motif-based biosensors intracellular applications.