Relationships between stress protein induction and NMDA-mediated neuronal death in the entorhinal cortex.

摘要: The entorhinal cortex (EC) appears to be one of the earliest regions express cellular pathology in aging and Alzheimer's disease. relationships between stress protein responses temporal spatial aspects cell death induced by N-methyl-D-aspartate (NMDA) was investigated this anatomical region. Low doses NMDA were infused stereotactically into medial EC rat. At intervals starting from 0.5 h up 7 days after a 1.25-μl infusion 15 mM NMDA, 30 or saline, expression ubiquitin (Ub), 72-kDa heat shock (HSP 72), c-Fos determined relation neuronal death. Volumes Ub- HSP 72-like immunoreactivity peaked 18 48 either infusions. After infusions, maximal volumes 72- Ub-like at similar subsequent volume loss seen 96 hours. final corresponded 70–80% HSP-Ub response 2 days, implying that population Ub-immunopositive cells survived insult. C-Fos as for nuclear phosphoprotein (Fos) indicated activation sites, perirhinal cortex, hippocampus, other sites throughout injected hemisphere. In EC, returned baseline levels 8 h, well before dramatic increases 72 Ub volumes. Our results demonstrate vivo precedes correlates with, but does not necessarily lead to, following glutamate receptor-mediated toxicity EC.