Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase.

摘要: Objective To explore the effects of 17β-estradiol (E 2 ) on cytoskeletal remodeling and motility endometrial stromal cells (ESC) Ishikawa to characterize role focal adhesion kinase (FAK) in these processes. Design In vitro study cellular morphology ESC or cells. Setting University research center. Patient(s) Endometrial samples obtained from women requiring biopsies. Intervention(s) Treatments with E multiple inhibitors signaling pathways. Main Outcome Measure(s) Activation FAK, actin remodeling, membrane morphology, cell motility, invasion. Result(s) Estrogen induces a rapid concentration-related FAK phosphorylation this time frame, localizes plasma at sites complexes formation, as shown by immunofluorescence. Phosphorylation presence estrogen depends recruitment both receptor α β G protein–dependent c-Src phosphatidylinositol 3-OH kinase. is required for estrogen-induced horizontal migration invasion three-dimensional matrices Conclusion(s) enhances controlling thus resulting enhanced These findings may have clinical relevance development new therapeutic strategies prevention control diseases.